Microbiology testing techniques

A. In the area where WHO will assign you, you will need to set up a field lab to investigate outbreaks. You will need to be able to identify the causal microbes in order to decide how to handle the outbreak. Discuss what do you need to know prior to going to the WHO site about techniques for identifying microbes?

B. Design and submit a concept map on what you have learned. It should include concepts and connections between concepts including:

  1. Microbiology testing techniques
  2. Types of microbes identified by each technique

C. Activity 1:Diagnosing Ebola – Laboratory Tests

Diagnosing Ebola is often difficult in the early stage, 2 -3 days after becoming infected, because the symptoms are the same as seen in other diseases of the area such as malaria. Diagnosis through testing is determine by a timeline of infection. Make a timeline graphic that shows how Ebola is diagnosed by tests. You will need to do research. You can complete this activity individually or with a team. Make the timeline graphic with pictures and descriptions.

Activity 2: Virtual Lab Gram Stain

Complete the Gram Stain virtual lab

Activity 3: Virtual Lab Bacteriology

Complete the virtual Bacteriology Lab

                            1. Gram Stain
                            2. Streak Plate
                            3. Differential Media
                            4. Biochemical Tests
                            5. Antimicrobial Susceptbility

Activity 4: Case Study

Complete the Case Study: The Case of the Newborn Nightmare

First, Download the case study.

Second, Work through the case, answering all questions.

D. Now that you have reviewed techniques for identifying infectious agents, and how to carry them out, what equipment and materials will you need to have for your field lab? Remember, supplies are delivered infrequently so you have to plan ahead to be sure you take everything you need. The supplies and equipment will need to work in remote areas where electricity is limited through use of a generator. Water will need to be boiled.

E. Design and submit a concept map on what you have learned. It should include concepts and connections between concepts including:

How genetics affects replication and growth of microbes by type.

F. Activity 1: MRSA

Antibiotic resistant strains of bacteria have become an increasing problem. Bacteria have the ability to change in order to survive. To see the effect this has had, work through the animation on MRSA

Now design a poster that could be used in a hospital or community center to inform people about MRSA and the need to complete antibiotics as ordered.

Activity 2: Microbial Genetics Lab

Complete the Virtual Genetics Lab

Investigate the games, activities and information to learn more about genetics at Genetics Alive

Activity 3: Case Study

Complete the case, The Cow of the Future, Genetically Engineering a Microbe to Reduce Bovine Methane Emissions

First, Download the case study.

Second, Work through the case, answering all questions.