PHOTOSYNTHESIS REPORT SHEET
PHOTOSYNTHESIS REPORT SHEET
Download the report sheet, complete it, and then submit it via Blackboard email as an attachment.Do not change the format.
All blue shaded areas require answers.
I. Using the spectroscope
Experiment (dry lab)
| View | List colors not absorbed by the filter/extract (colors you see) |
| Red filter | |
| Blue filter | |
| Green filter | |
| Spinach extract |
| What is the relationship between the color of the filter and that filter’s absorption of light? |
| Both the spinach extract and the green filter are green in color. How does the spinach extract compare to the absorption spectrum of the green filter? |
II. Absorption spectrum
Experiment (dry lab)
| 400 nm | 425 | 450 | 475 | 500 | 525 | 550 | 575 | 600 | 625 | 650 | 675 | 700 | |
| Carrot extract | |||||||||||||
| Spinach extract |
Absorption Spectrum Graph for carrot extract (orange line) and spinach extract (green line), superimposed.
(Fill table using Microsoft insert shapes or manually graph, scan, embed image into report.)
| What wavelengths and colors of light are not absorbed well (reflected/transmitted) by the carrot extract (carotenoids)? |
| What wavelength and colors of light is not absorbed well (reflected/transmitted) by the spinach extract (chlorophylls mainly)? |
III. Pigment chromatography
Experiment (dry lab)
| Which pigment has the greatest affinity for the solvent (is most soluble in the solvent and travels right along with the solvent)? | |
| Which pigment has the greatest affinity for the paper (is most attracted to the paper)? |
| Distance traveled by the solvent in cm | 54 mm = 5.4 cm |
| Pigment color | Distance traveled in cm (from baseline to pigment band) | *Rf value
(pigment distance divided by solvent distance) |
|
| carotenes | Orange | 4.8 cm | 4.8/5.4 = 0.89 |
| xanthophylls | |||
| chlorophyll a | |||
| chlorophyll b |
| What pigment do you think is present in the highest concentration during the spring season when many leaves of deciduous plants are green? |
| In the fall, leaves turn red, orange, yellow, and eventually brown. Where did the green color go? |
IV. Starch localization
Experiment (dry lab)
| What would be your hypothesis on the localization of starch? | |
| Does this correspond to the localization of chlorophyll pigments? | |
| Which part of the leaf stores starch?
(darker outer region or lighter inner region) |
V. Chloroplast structure
Experiment (dry lab)
Match the descriptions to the labels below
| a.site for light reactions, electron transport chain and ATP-synthesizing enzymes | |
| b.Site for Calvin cycle | |
| c.Surrounds the organelle |
| Thylakoid
Stroma Outer membrane |
CELLULAR RESPIRATION REPORT SHEET
Download the report sheet, complete it, and then submit it via Blackboard email as an attachment.Do not change the format.
All blue shaded areas require answers.
- Oxygen Consumption
Experiment (dry lab)
| Organism type (common name) | |
| Organism mass (g) |
| Trial No. | Volume O2 used (ml) | Time interval
(seconds) |
Time interval
(hour) |
ml O2/ g / hr |
| 1 | 2ml | 150 | 0.042 | 2.11 |
| 2 | 2ml | 180 | ||
| 3 | 2ml | 175 | ||
| average | 2ml |
Plot a graph relating metabolic rate and mass.
| Animal | Metabolic rate(ml O2/g/hr) | Approximate mass |
| Kangaroo mouse | 1.8 | 0.02 kg |
| Rat | 0.87 | 1 kg |
| Cat | 0.68 | 2 kg |
| Dog | 0.33 | 7 kg |
| Man | 0.21 | 70 kg |
| Elephant | 0.07 | 1000 kg |
(Fill table using Microsoft insert shapes or manually graph, scan, embed image into report.)
| As body mass increases, oxygen consumption ____. |
II. CO2 production in plant embryos
Experiment (dry lab)
| seeds | Phenol redcolor at 0 min (initial color) | Phenol red color at 60 min (red, yellow, or fuchsia) | Was there a change in pH?
(yes or no) |
Is CO2present or absent? |
| Bottle #1:
(GERM) |
Red | |||
| Bottle #2:
(GERM – Boil) |
Red |
| Color of 5 ml of cabbage juice | |
| Color of 5 ml of cabbage juice after blowing CO2 bubbles |
| seeds | Cabbage juice color at 0 min | Cabbage juice color at 60 min (lighter, darker or same color) | Was there a change in pH?
(yes or no) |
Is CO2present or absent? |
| Jar #1:
germinating (boiled) |
Violet | |||
| Jar #2:
germinating (not boiled) |
Violet |
III. The mitochondrion
Experiment (dry lab)
Match the descriptions to the labels below.
| a. CO2 production | |
| b.Site for electron transport chain and ATP-synthesizing enzymes | |
| c.Surrounds the organelle | |
| d.Compartment between outer and inner membranes |
|
IV. Fermentation in yeast
Experiment (at home lab)
Record the amount of CO2 (as none, some or most).
| Container # | Amount of sucrose | Estimate amount of CO2 at 0 minutes | Estimate amount of CO2 at 12 hours (none, some or most) |
| 1 | 1 teaspoon | 0 | |
| 2 | 10 teaspoons | 0 | |
| 3 | 0 | 0 |
| QUESTIONS: |
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